We combined this property with a target-labelling protocol that preserves the original polarity of the transcripts with a view to determining the differential expression of non-annotated transcriptionally active regions.
A significant advantage of TMAs over EMAs is the possibility of detecting non-annotated transcripts generated only under specific physiological conditions. The results afforded a Pearson correlation coefficient of 0.943, indicating that TMAs are as reliable as EMAs for quantitative expression analysis. We experimentally tested the potential of our approach by measuring the differential expression of 4904 genes in the yeast Schizosaccharomyces pombe growing under conditions of oxidative stress. pombe 1.0FR tiling microarrays to generate custom Chip Description Files (CDF) in order to compare the efficiency of both platforms. We extensively filtered probes present in Affymetrix Genechip Yeast Genome 2.0 expression and GeneChip S.
The purpose of this study was to develop a protocol that would take advantage of the high resolution of TMAs for quantitative measurement of DNA strand-specific differential expression of annotated and non-annotated transcripts. By contrast, tiling microarrays (TMA) have a much higher probe density and provide unbiased genome-wide coverage.
Expression microarrays (EMA) contain probes for annotated open reading frames (ORF) and are widely used for the analysis of differential gene expression. GEO help: Mouse over screen elements for information.Īnalysis of DNA strand-specific differential expression with high density tiling microarraysĮxpression profiling by genome tiling arrayĭNA microarray technology allows the analysis of genome structure and dynamics at genome-wide scale.
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